Sweetfuel Project Methodology Breeding for low temp.
The general objective of this WP is to develop sorghum hybrids enhanced for yield and composition of biomass and better adapted to temperate environments in order to satisfy the bio-fuel policy of Europe in benefit of its farmers and agro-industrials and in respect of environmental concerns.
1. Develop an ideotype concept for biomass based, bio-ethanol sorghum combining desirable trait combinations for temperate climates.
2. Establish a shared germplasm working collection and screen it for cold tolerance, drought tolerance, biomass yield and composition of biomass.
3. Develop an economical and rapid method to characterize the chemical composition of sorghum biomass with respect to enzymatic digestibility.
4. Identify/breed fertility restorers and male sterile lines carrying abiotic stress tolerance and biomass quality traits.
5. Develop new biomass sorghum hybrids based on the restorers and male sterile lines generated.
6. Investigate the genome location of genetic factors (QTLs and genes) of traits involved in biomass quality (bmr trait) and cold tolerance using molecular markers.
7. Develop new sorghum experimental genotypes by MAS activities focused on the transfers of bmr genes and QTLs pyramiding for cold tolerance.
8. Provide stakeholders in Europe and other temperate environments with sorghum biomass hybrids that are well-documented, well-tested in multi-location trials and well-adapted to the requirements of 2nd-generation ethanol production technologies.
Task 1.1. Establishment of working collection, exchange of germplasm and seed increase
Task 1.2. Evaluation and selection of germplasm
Task 1.3. Development of hybrids
Task 1.4. Molecular-genetic characterization and MAS of key traits
Task 1.5. Final evaluation of breeding products
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